THE EFFECT OF ELECTROMAGNETIC RADIATION (EMR) FROM LAPTOP ON REPRODUCTIVE HORMONES, SPERM QUALITY AND PROSTATE SPECIFIC ANTIGEN OF MALE ALBINO RATS (RATTUS NORVEGICUS)
Humans in modern society are exposed to an ever-increasing number of electromagnetic fields (EMFs) generated from the production and supply of electricity, television sets, personal computer (PC), radio communication, and mobile communication, hence, it has become a public health issue. This study was conducted to evaluate the effects of electromagnetic radiation (EMR) emitted from new generation laptop computers on sperm quality and reproductive hormone of male albino rats. Male albino rats (10-12 weeks old) were exposed to RF-EMR from laptop computers which were grouped based on different exposure period (2hours, 4hours, 6hours and 8hours) for 4 weeks. The semen samples were obtained by caudal puncture of the epididymis from each participant for sperm quality analysis and blood collected by cardiac puncture for hormonal analysis using the chemiluminescent microparticles immunoassay method. The analysis of variance was done for the hormonal concentration and sperm quality parameters to check for the significance difference at 5% level of significance. The Dunnett’s multiple comparison test was done to test for significance comparison of radiation exposed groups and control group. Exposure to laptop computer display unit was associated with significant reduction in sperm motility, sperm viability and sperm count (P<0.0001), testosterone level (P<0.001), follicle stimulating hormone level (P<0.01). For the sperm morphology, there was no significant difference in the normal cells for the experimental setup, however, the appearance of abnormal cells in the exposed rats (2 – 8 hrs) were significant (P≤0.001). This study therefore showed that EMR from a charging laptop can significantly affect semen quality, male fertility and rendered male reproductive hormone unstable with no effect on prostrate specific antigens.
Adams, A.J., Tamara S.G., Debapriya M.S., and Fiona, M. (2014). Effect of mobile telephones on sperm quality: A systematic review and meta-analysis. Environment International, 70: 106–112.
Agarwal, A., Desai, N.R., Ruffoli, R., and Carpi, A. (2008). Lifestyle and testicular dysfunction: a brief update. Journal of Biomedicine Pharmacotherapy; 62:550–553.
Aitken, R.J., Wingate, J.K., De Iuliis, G.N., Koppers, A.J. and McLaughlin, E.A. (2006). Cis unsaturated fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa. Journal of Clinical and Endocrinology Metabolism; 91: 4154–4163.
Asghari, A., Khaki, A.A., Rajabzadeh, Khak, A. (2016). A review on Electromagnetic fields (EMFs) and the reproductive system. Electron Physician, 8(7): 2655–2662.
Baan, R., Gross, Y., Lauby-Secretan, B., El Ghissassi, F., Bouvard, V., Benbrahim-Tallaa, L., et al. (2011). Carcinogenicity of radiofrequency electromagnetic fields. Lancet Oncol. 12:624–626.
Basso, D., Guariso, G., Fasolo, M., Pittoni, M., Schiavon, S., Fogar, P., Greco, E., Navaglia, F., Zambon, C.F. and Plebani, M. (2006). A new indirect chemiluminescent immunoassay to measure anti tissue trans glutaminase antibodies. Journal of Pediatric Gastroenterology and Nutrition; 43(5): 613 – 618.
Bergendahl, M., Evans, W.S., and Veldhuis, J.D. (1996). Current concepts on ultradian rhythms of luteinizing hormone secretion in the human. Hum. Reprod. Update, 2: 507-518.
Björndahl, L., Soderlund, I., and Kvist, U. (2003). Evaluation of the one-step eosin-nigrosin staining 8858 technique for human sperm vitality assessment. Journal of Human Reproduction, 18: 813–816.
Conrado, M.S., Ariela, M.S., Cesar, A., Sanchez, M.D., Gustavo, F., and Doncel, M.D. (2012). Effects of laptop computers connected to local area networks wirelessly (Wi-Fi) on human spermatozoa. Journal of Andrology, 97(1): 39-45.
Cuccurazzu, B., Leone, L., Podda, M.V., Piacentini, R., Riccardi, E. and Ripoli, C. (2010). Exposure to extremely low-frequency (50 Hz) electromagnetic fields enhances adult hippocampal neurogenesis in C57BL/6 mice. Journal of Experimental Neurology, 226: 173–182.
De luliis, G.N., Newey, R.J., King, B.V., and Aitken, R.J. (2009). Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa In Vitro. PLoS One, 4(7): e6446.
De Oliva, S.U., and Miraglia, S.M. (2009). Carbamazepine damage to rat spermatogenesis in different sexual developmental phase. International Journal Andrology, 32:563-574.
Feychting, M., Ahlbom, A. and Kheifets, L. (2005). EMF and health. Journal of Annual Review of Public Health, 26:165-189.
Gholampour, F., Owji, S.M., Javadifar, T.S., and Bahaoddini, A. (2012). Long term Exposure to Extremely Low Frequency Electromagnetic Field Affects Sex Hormones Level and Structure of Testis in Rats. International Journal of Zoological Research, 8: 130-136.
Gollenberg, A.L., Liu, F., Brazil, C., Drobnis, E.Z. and Guzick, D. (2010). Semen quality in fertile men in relation to psychosocial stress. Journal of Fertility and Sterility, 93: 1104–1111.
Gye, M.C., and Park, C.J. (2012). Effect of electromagnetic field exposure on the reproductive system. Clin Exp Reprod Med. 39(1): 1-9
Harrington, J.M., McBride, D.I., Sorahan, T., Paddle, G.M., and van Tongeren, M. (1997). Occupational exposure to magnetic fields in relation to mortality from brain cancer among electricity generation and transmission workers. Occup Environ Med. 54(1):7–13.
Isojarvi, J.I., Lofgren, E., Jantunen, K.S., Pakarinen, A.J, Paivansalo, M., Rautakorpi, I. and Tcomivaraara, L. (2004). Effect of epilepsy and antiepileptic drugs on male reproductive health. Journal of Neurology, 62: 247-253.
Khayyat, L.I. (2011). The histopathological effects of an electromagnetic field on the kidney and testis of mice. EurAsia J. Biosci. 5: 103-109.
Koppers, A.J., De Iuliis, G.N., Finnie, J.M., McLaughlin, E.A., Aitken, R.J. (2008). Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab. 93: 3199–3207.
Lewis, S.E., Aitken, R.J. (2005). DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 322: 33–41.
Mailankot, M., Kunnath, A.P., Jayalekshmi, H., Koduru, B., and Valsalan, R. (2009). Radio Frequency Electromagnetic Radiation (RF-EMR) from GSM (0.9/1.8GHZ) Mobile Phones Induces Oxidative Stress and Reduces Sperm Motility in Rats. Clinics (Sao Paulo) 64(6): 561–565.
Margonato, V., Veicsteinas, A., Conti, R., Nicolini, P., and Cerretelli, P. (1993). Biologic effects of prolonged exposure to ELF electromagnetic fields in rats. I.50 Hz electric fields. Bioelectromagnetics, 14: 479-493.
Mashevich, M., Folkman, D., Kesar, A., Barbul, A., Korenstein, R., Jerby, E., et al. (2003). Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability. Bioelectromagnetics, 24:82–90.
Meo, S.A., Al-Drees, A.M., Husain, S., Khan, M.M., and Imran, M.B. (2013). Effects of mobile phone radiation on serum testosterone in wistar albino rats. Saudi Med Journal, 31(8): 863-873.
Oer, M., Beckert, C., Heinrich, B., Buenning, C., and Kapprell, H. (2012). Performance evaluation of an improved architect lh assay for the quantitative determination of luteinizing hormone in human serum and plasma. Biology, 2: 27-36.
Otitoloju, A.A., Obe, I.A., Adewale, O.A., Otubanjo, O.A., and Osunkalu, V.O. (2010). Preliminary study on the induction of sperm head abnormalities in mice, Mus musculus, exposed to radiofrequency radiations from global system for mobile communication base stations. Bull Environ Contam Toxicol. 84:51–54.
Ozguner, M., Koyu, A., Cesur, G., Ural, M. and Ozguner, F. (2005). Biological and morphological effects on the reproductive organs of rats after exposure to to electromagnetic field. Saudi Med Journal, 26(3): 405-410.
Pant, N., Pant, A., Shukla, M., Mathur, N., Gupta, Y. and Saxena, D. (2011). Environmental and experimental exposure of phthalate esters: the toxicological consequence on human sperm. Journal of Human and Experimental Toxicology, 30: 507–514.
Rajaei, F., Mahdi, F., Ghasemi, N., Sarreshtehdari, M., Gheybi, N.A., and Saraeisahneh, M.S. (2009). Effect of electromagnetic field on mice epididymis and vas deferen: A morphometric study. J. Gorgan Univ. Med. Sci., 11: 1-7.
Rolland, M., Le Moal, J., Wagner, V., Royere, D. and De Mouzon, J. (2013). Decline in semen concentration and morphology in a sample of 26,609 men close to general population between 1989 and 2005 in France. Journal of Human Reproduction, 28: 462–470.
Rouge M. (2004). Sperm morphology assay: Hypertexts for biomedical sciences.Colorado State University Press, Colorado. Pp 33-36.
SCENIHR - Scientific Committee on Emerging and Newly Identified Health Risks (2015). Opinion on potential health effects of exposure to electromagnetic fields. Bioelectromagnetics, 36(6): 480-484.
Scott, A. (2007). Architect system hormonal assay procedure. Abbott laboratories diagnostic division Abbott park, IL USA. Pp 1-5.
Sobel, E., Dunn, M., Davanipour, Z., Qian, Z., and Chui, H.C. (1996). Elevated risk of Alzheimer’s disease among workers with likely electromagnetic field exposure. Neurology, 47(6):1477–81
Sonmez, M., Yuce, A. and Turk, G. (2007). The protective effects of melatonin and Vitamin E on antioxidant enzyme activities and epididymal sperm characteristics of homocysteine treated male rats. Reproductive Toxicology, 23: 226–231.
Sykes, P.J., McCallum, B.D., Bangay, M.J., Hooker, A.M., and Morley, A.A. (2001). Effect of exposure to 900 MHz radiofrequency radiation on intrachromosomal recombination in pKZ1 mice. Radiat Res. 156:495–502.
Tremellen, K. (2008). Oxidative stress and male infertility – a clinical perspective. Journal of Human Reproduction, 14:243–258.
Verma, R.J. and Chinoy, N.J. (2002). Effect of carica papaya seeds extract on male albino rats. Asian Journal of Andrology, 4(1):77-78.
Volkow, N.D., Tomasi, D., Wang, G.J., Vaska, P., Fowler, J.S., and Telang, F. (2011). Effects of cell phone radiofrequency signal exposure on brain glucose metabolism. Journal of the American Medical Association; 305: 808–813.
Warita, K., Sugawara, T., Yue, Z.P., Tsukahara, S., and Mutoh, K.J. et al., (2006). Progression of the dose-related effects of estrogenic endocrine disruptors, an important factor in declining fertility, differs between the hypothalamo-pituitary axis and reproductive organs of male mice. J. Vet. Med. Sci., 68: 1257-1267.
World Health Organization. (2010). WHO research agenda for radio frequency fields. Available at: http://whqlibdoc.who.int/publications/2010/9789241599948_eng.pdf. Accessed on March 13, 2018.
Yan, M.D., Michael, M.S., Tim Bruce, B.S., Yu Hui Yan, M.D., Amy, B.S., Hani, S. and Matloub, M.D. (2007). Effects of cellular phone emissions on sperm motility in rats. Journal of Fertility and Sterility, 88: 957–964.
Zaneveld, L.D. Polakoski, K.L. (1977). Collection and physical examination of ejaculate. In: Hafez E.S.E, ed. Techniques of Human Andrology. Amsterdam, Holland: North Holland Biomedical Press.147-156.
Zhang, X.Z., Liu, J.H., Sheng, H.Q., Wu, H.J., and Wu, Y. (2013). Seasonal variation in semen quality in China. Journal of Andrology,1: 639–643.